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<t>Tgr5</t> mRNA (A) expression in liver of myeloid-specific Fmr1 deficient (Fmr1 fl -LysM cre ) compared to control (Fmr1 fl ), N = 5 each. Representative Western blot (B) of Tgr5 from liver extract of Fmr1 fl -LysM cre and Fmr1 fl mice with quantification below, N = 4-5 each. IL-1β (C) and IL-6 (D) mRNA expression in livers (N = 4-5 each). (A-D) *P < 0.05 ANOVA with multiple comparison test. BMDM Tgr5 mRNA expression in male; N = 4-5 each (E) and Western blot with quantification below (F) in both female (N = 2 each) and male (N = 2-3) Fmr1 fl compared to Fmr1 fl -LysM cre ; **P < 0.01 T test. Gating scheme (G) for Tgr5 staining and FACS analysis of peritoneal macrophage double positive for F4/80 and CD11b. Percentage of Tgr5 + peritoneal macrophage (H) of Fmr1 fl (N = 8; 4 each sex) compared to Fmr1 fl -LysM cre (N = 6; 3 each sex) mice. *P < 0.05 T test.
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<t>Tgr5</t> mRNA (A) expression in liver of myeloid-specific Fmr1 deficient (Fmr1 fl -LysM cre ) compared to control (Fmr1 fl ), N = 5 each. Representative Western blot (B) of Tgr5 from liver extract of Fmr1 fl -LysM cre and Fmr1 fl mice with quantification below, N = 4-5 each. IL-1β (C) and IL-6 (D) mRNA expression in livers (N = 4-5 each). (A-D) *P < 0.05 ANOVA with multiple comparison test. BMDM Tgr5 mRNA expression in male; N = 4-5 each (E) and Western blot with quantification below (F) in both female (N = 2 each) and male (N = 2-3) Fmr1 fl compared to Fmr1 fl -LysM cre ; **P < 0.01 T test. Gating scheme (G) for Tgr5 staining and FACS analysis of peritoneal macrophage double positive for F4/80 and CD11b. Percentage of Tgr5 + peritoneal macrophage (H) of Fmr1 fl (N = 8; 4 each sex) compared to Fmr1 fl -LysM cre (N = 6; 3 each sex) mice. *P < 0.05 T test.
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<t>Tgr5</t> mRNA (A) expression in liver of myeloid-specific Fmr1 deficient (Fmr1 fl -LysM cre ) compared to control (Fmr1 fl ), N = 5 each. Representative Western blot (B) of Tgr5 from liver extract of Fmr1 fl -LysM cre and Fmr1 fl mice with quantification below, N = 4-5 each. IL-1β (C) and IL-6 (D) mRNA expression in livers (N = 4-5 each). (A-D) *P < 0.05 ANOVA with multiple comparison test. BMDM Tgr5 mRNA expression in male; N = 4-5 each (E) and Western blot with quantification below (F) in both female (N = 2 each) and male (N = 2-3) Fmr1 fl compared to Fmr1 fl -LysM cre ; **P < 0.01 T test. Gating scheme (G) for Tgr5 staining and FACS analysis of peritoneal macrophage double positive for F4/80 and CD11b. Percentage of Tgr5 + peritoneal macrophage (H) of Fmr1 fl (N = 8; 4 each sex) compared to Fmr1 fl -LysM cre (N = 6; 3 each sex) mice. *P < 0.05 T test.
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Tgr5 mRNA (A) expression in liver of myeloid-specific Fmr1 deficient (Fmr1 fl -LysM cre ) compared to control (Fmr1 fl ), N = 5 each. Representative Western blot (B) of Tgr5 from liver extract of Fmr1 fl -LysM cre and Fmr1 fl mice with quantification below, N = 4-5 each. IL-1β (C) and IL-6 (D) mRNA expression in livers (N = 4-5 each). (A-D) *P < 0.05 ANOVA with multiple comparison test. BMDM Tgr5 mRNA expression in male; N = 4-5 each (E) and Western blot with quantification below (F) in both female (N = 2 each) and male (N = 2-3) Fmr1 fl compared to Fmr1 fl -LysM cre ; **P < 0.01 T test. Gating scheme (G) for Tgr5 staining and FACS analysis of peritoneal macrophage double positive for F4/80 and CD11b. Percentage of Tgr5 + peritoneal macrophage (H) of Fmr1 fl (N = 8; 4 each sex) compared to Fmr1 fl -LysM cre (N = 6; 3 each sex) mice. *P < 0.05 T test.

Journal: PLOS One

Article Title: Myeloid Fmr1 deficiency in mice results in reduced serum cholesterol and altered bile pathway gene expression

doi: 10.1371/journal.pone.0340222

Figure Lengend Snippet: Tgr5 mRNA (A) expression in liver of myeloid-specific Fmr1 deficient (Fmr1 fl -LysM cre ) compared to control (Fmr1 fl ), N = 5 each. Representative Western blot (B) of Tgr5 from liver extract of Fmr1 fl -LysM cre and Fmr1 fl mice with quantification below, N = 4-5 each. IL-1β (C) and IL-6 (D) mRNA expression in livers (N = 4-5 each). (A-D) *P < 0.05 ANOVA with multiple comparison test. BMDM Tgr5 mRNA expression in male; N = 4-5 each (E) and Western blot with quantification below (F) in both female (N = 2 each) and male (N = 2-3) Fmr1 fl compared to Fmr1 fl -LysM cre ; **P < 0.01 T test. Gating scheme (G) for Tgr5 staining and FACS analysis of peritoneal macrophage double positive for F4/80 and CD11b. Percentage of Tgr5 + peritoneal macrophage (H) of Fmr1 fl (N = 8; 4 each sex) compared to Fmr1 fl -LysM cre (N = 6; 3 each sex) mice. *P < 0.05 T test.

Article Snippet: Primary antibodies used were: LDLR (Invitrogen), Cyp7b1 (Proteintech), Cyp27a1 (Abcam) and Tgr5 (Proteintech).

Techniques: Expressing, Control, Western Blot, Comparison, Staining